Pcr Applications Protocols For Functional Genomics PDF, ePub eBook
Pcr Applications Protocols For Functional Genomics
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Polymerase chain reaction pcr is a technique used in molecular biology to amplify a single copy or a few copies of a segment of dna across several orders of magnitude generating thousands to millions of copies of a particular dna sequence. Figure 1 two step assembly pcr step 1 oligonucleotides with overlapping sequences approximately 20 bp and with similar tm are assembled into double stranded dna 500 1000 bp using pcr. Reverse transcription polymerase chain reaction rt pcr a variant of polymerase chain reaction pcr is a technique commonly used in molecular biology to detect rna expression
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